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Captive breeding of loach minnow, a threatened species in Arizona
 
Male loach minnow in breeding colors.Background:
Loach minnow is a small secretive fish that is native to the Gila River Basin in Arizona, New Mexico, and Sonora, Mexico. Historically, the species was locally abundant, but populations have declined drastically in recent years, and the species is now restricted to sparse tributary populations in Arizona and New Mexico. The species is listed as threatened by the U.S. Fish and Wildlife Service and is listed as a species of special concern in Arizona and New Mexico. Development of captive propagation techniques for loach minnow has been proposed as a means to facilitate species recovery. The Research Branch began a study in 2002 to induce adult loach minnow to spawn in the laboratory, and to develop and document conditions necessary for incubation and hatching of their eggs, as well as the rearing of larvae, juveniles, and adults.

Location:
Loach minnow were collected from Aravaipa Creek, Arizona, in the fall of 2002. Fish were captured by seining riffles, and fish were transported by hatchery truck to Bubbling Ponds Hatchery, near Cornville, Arizona. Fish are being held indoors in recirculating aquaria, where water temperature is controlled by room temperature, and an electronic timer and full-spectrum fluorescent light fixtures control lighting.

Approach:
Aravaipa Creek, Arizona. Spawning in raceways is elicited by manipulation of water temperature and photoperiod to mimic conditions associated with natural spawning of wild fish. In the laboratory, day length is set to mimic April by means of an electronic timer, and loach minnow are held in raceways at an ambient room temperature of 21 C. Flow velocities are approximately 10 cm/sec through the recirculating system. Spawning substrate consists of small cobbles and broken pieces of clay pots. Fish are fed twice daily with a combination of commercial flake food and freeze-dried bloodworms.

Eggs are collected twice each day to protect them from cannibalism by adults, and are incubated at 21 C. The eggs hatch after about 1- 4 days, and larval fish are quickly moved to holding aquaria, where they are provided starter foods, such as live brine shrimp and commercial larval fish diets. As fish grow, food size is increased slowly for about 4 weeks, until the fish are large enough to ingest commercial flake foods and freeze-dried bloodworms. Fish tanks must be kept clean, and fish must be monitored daily for signs of disease. Future research will include hormone injections to induce spawning, and controlled breeding experiments to determine optimal male:female ratios among spawning adults. The final report for this project is due September 30, 2004.

Benefits:
The ultimate goal of this study is to provide hatcheries with the techniques necessary to maintain loach minnow broodstock, and the ability to produce progeny for future restocking into the wild. This will help meet the goals of the loach minnow Recovery Plan.

For more information contact:
Mike Childs, Arizona Game and Fish Department, 1600 North Page Springs Road, Cornville, AZ 86325.
Phone: (928) 639-1346. E-mail: mike@sedona.net
 
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